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Questions related from Alison Catalano
The standard curve reagent in our validated assay is a polyclonal antibody. It is used to measure a drug substance that is also a polyclonal antibody. This is not measuring like we would like,...
18 October 2016 5,974 6 View
When developing a competitive ELISA how do you improve sensitivity? The format is to measure polyclonal antibody: a capture mAB on the plate, captures antigen, and then a labelled mAB is...
21 June 2016 8,293 10 View
I have been trying to develop an assay to measure recombinant hylauronidase activity. It is a chromogenic method which measures N-acetylglucosamone (NAG) production by hyaluronidase on hyaluronic...
19 January 2015 9,915 1 View
When developing ELISA I have always followed the rule of coating just below saturation level. This avoids stacking issues. I would like to know if there are any consequences for using lower...
03 June 2014 5,340 10 View
