Alison Blain

3 Questions 6 Answers 0 Followers

Questions related from Alison Blain

Anyone have an automated method for counting cells/determining cell density on stained brain slices?

I have cresyl violet stained brain sections and would like to look at cell density in different regions of the brain including the hippocampus and areas of the motor cortex. In some places the...

12 May 2014 8,717 7 View

Is it possible to 'over transfer' proteins from a gel to a membrane (western blotting)?

We were wondering if it is worth transferring a very large protein overnight. I was always led to believe that your proteins can migrate all the way through the membrane and be lost but someone...

12 February 2014 5,728 26 View

ELISA works for spiked TE buffer but not spiked tissue lysate- potential ways to fix this?

I suspect proteins in the lysate are interfering with antibody binding.

21 January 2014 1,271 2 View