Alireza Sazmand

2 Questions 6 Answers 0 Followers

Questions related from Alireza Sazmand

Does clearing of filarial nematodes for identification affects PCR? What about clearing vs SEM?

We have limited number of filarial worms in 70% ethanol. First I should identify the nematodes and their sex, then perform either SEM or PCR. Does clearing (lactophenol) will affect the quality of...

04 April 2016 4,470 6 View

Does the TCA precipitation of protein antigens interfere with indirect-ELISA?

I'm developing an ELISA for coccidian parasite Cystoisoispora. I'm wondering if I precipitate proteins of sporozoite/merozoite lysate by TCA I can be sure of quality or I'd better use other...

07 July 2014 9,827 6 View