My Strep-tagged protein doesn`t bind IBA Tactin XT 4Flow spin columns. Could you please advice how to solve the problem with bindning to resin?

My protein is UFM1 tagged with Twin-Strep tag. I double-checked and my tag is not cleaved off or hidden. I made purification with IBA kit (Tactin XT 4Flow high capacity spin kit) with subsequent...

27 November 2023 4,067 3 View