How to adjust primers for multiplexing using a universal tag based 3-primer (per locus) system?

I’m currently trying to develop a 10 SSR marker multiplex for allelic screening using the universal tags and the method (iii) from Blacket et al. (2012 - DOI: 10.1111/j.1755-0998.2011.03104.x )....

15 March 2021 3,057 1 View