3 Questions 5 Answers 0 Followers
Questions related from Alice Coburn
To prepare for an assay where several plasmids need to be transfected together, I am trying to optimize transfection into equine dermal fibroblasts with a constitutive GFP plasmid. I've tried...
03 March 2014 1,461 3 View
I'm trying to ligate a 2 kb insert into a 3.5kb plasmid, which normally works ok. I transform into top10 competent cells, plate & have colonies the next day. I do colony PCR to check whether my...
03 March 2014 9,044 3 View
I have a package of cells being shipped to the UK from the States, which is currently being held in customs. How long can the cell vial survive on the dry ice? It will be at least 4 days from...
01 January 2014 258 4 View
