Alice Nardin

2 Questions 3 Answers 0 Followers

Questions related from Alice Nardin

Surface Plasmon resonance (SPR) protein not captured by chip?

Hi, I'm having troubles with an SPR experiment. I'm isolating my protein which has a (2x)HIS-FLAG-tag from mammalian cells (HEK293) and a mw around 150kDA. I've been using both Ni-NTA beads and...

12 September 2019 1,726 5 View

(2x)His-tagged protein doesn't bind to Ni-NTA chip for SPR experiment. suggestions?

Hello, I am having troubles with an SPR experiment. The protein I want to conjugate to the chip has a double his-flag-tag at its N-terminus. I overexpress the protein on HEK293 and then IP with...

16 July 2019 6,820 3 View