Why does Nocodazole work poorly in Saccharomyces cerevisiae under heat stress conditions?

I am trying to arrest budding yeast cells at 30°C and 42°C. While at 30°C after 1.5 h with 10 ug/ml Nocodazole I observe a population of metaphase-arrested cells, at 42°C I see cells at various...

15 November 2019 1,032 3 View