Alia Mallah

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Questions related from Alia Mallah

Can someone suggest a detailed protocol for aggrecan extraction from articular cartialge tissue?

I am looking for an easy to follow protocol on extracting aggrecan from articular cartilage tissue. Most papers cite the CsCl density gradient centrifugation purification method, but some of the...

13 March 2019 733 0 View

Hello does anyone have experience with using the LIVE/DEAD Viability/Cytotoxicity Kit *for mammalian cells from Thermofisher?

It's Calcein AM / Ethidium homodimer-1 stains. I stained chondrocytes and the images I got show that the Calcein AM stained all the cells, even the ones stained with the Ethidium Homodimer-1. I...

20 April 2017 6,370 4 View