Ali Farhoudi

3 Questions 8 Answers 0 Followers

Questions related from Ali Farhoudi

How can I get rid of DNA contamination after in vitro transcription?

I want to detect minus strand of HCV and I need a control. I tried to use in vitro transcription of my control plasmid for plus and minus strand control. However, even after two times of Turbo...

04 April 2013 5,925 2 View

Why did our plasmid yield (16kb) which was transformed into bacteria decrease significantly when we used a bacterial glycerol stock?

We make VLP (virus like particles) and we need to use pNL4,3 E-R- Luc+. We have a problem with this plasmid and every time that we use transformed bacterial glycerol stock to extract the plasmid...

02 February 2013 3,429 12 View

Is it possible to obtain a positive PCR result from RNA fragment without making cDNA?

For in vitro transcription we normally use high concentrations of our linear DNA. After double digestion with Dnase I and two times RNA purification, the PCR on the RNA is still positive even with...

09 September 2012 8,643 4 View