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I have accidently added ripa in the cytosolic protein. How can I re-concentrate my protein sample? I was fractioning the mammalian nuclear and cytosolic proteins.
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I am working with ligand conjugation with proteins. I want to conjugate my protein with ligand via cysteine residue. I have computed the surface area accessibility score of all cysteines. How can...
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I want to amplify the human beta globin 3' UTR from Human genomic DNA. I have done gradient PCR. But could not get my desired product. The Tm of Forward primer is 66 Celsius and Reverse Primer is...
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I have forward primer and reverse primer with 57 nucleotides. My 5' UTR is in these primer. I want to ligate these two ss primers. Is primer dimer formation gives me the final ds DNA or I would...
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