Ali Calderon-Aparicio

3 Questions 2 Answers 0 Followers

Questions related from Ali Calderon-Aparicio

How can I calculate the Ic50 for radiation in cell cultures?

I am new in the radiotherapy field and I need to set some experiments to determine the sensitivity of several cells lines to radiation. I read a lot of papers and they look like to achieve my goal...

20 January 2023 2,122 4 View

Hi. I forgot to add SDS to my running buffer in my western blot experiment, I made the transfer because are imp sampleCan I still save my experiment?

Samples are important, so I decided to go forward with my experiment. The Running buffer was used without SDS. Can I save my experiment t?

10 January 2021 2,664 4 View

Hello.. I need to isolate nuclear proteins and then do ImmunoPrecip to see interacting proteins using RCC2 as bait. Do you have any good lysis buffer?

I need to find non-known proteins that can bind RCC2? So, I need to do immunoprecipitation of RCC2 and then try IP assays. RCC2 is a nuclear protein, so I need to isolate RCC2 and its partners...

18 June 2018 8,520 2 View