Alfonso Blázquez Moreno

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Questions related from Alfonso Blázquez Moreno

Can I extract mRNA from fixed cells?

I want to sort my cells using a marker that require intracellular staining and so, fixation and permeabilation. After that staining, and sorting, can I use these cells as source for mRNA extraction?

11 November 2016 9,563 1 View

How long do T cells need to be activated by CD3-AB for phospho-Tyr measurement?

I am going to measure T cell activation in different conditions using Anti-Phosphotyrosine by Flow cytometry. I am going to inmovilized Ab-CD3 on wells, put my T cells on it and then stain them...

01 January 2015 3,661 4 View