How to electroporate RNP complexes (Nickase D10A and 2 sgRNA) (Crispr-Cas9) ?

Hello, We wish to electroporate ES cells with protein nickase D10A and two sgRNAs targeting our gene of interest. Before adding the RNP complexes to the electroporation cuvette containing our...

27 April 2020 9,081 0 View