Alexis Bonfim-Melo

2 Questions 8 Answers 0 Followers

Questions related from Alexis Bonfim-Melo

What is the best siRNA designer tool (for shRNA)?

I am currently designing oligo sequences for depletion by shRNA. I have found a lot of on line tools (below) but each one give me different oligo sequences and I just do not know which I should...

13 September 2012 7,978 6 View

Could we use the DH5alpha instead of Stbl3 for lentiviral plasmid transformation and cloning?

I will perform some transduction with lentivirus produced in our lab (not commercial made). At Addgene they say that the plasmid that I've got should be transfomed into Stbl3, however I was...

15 August 2012 7,069 42 View