Alexia Bosancic

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Questions related from Alexia Bosancic

Any help with mutation detection H63D Gel Electrophoresis Results?

Hello, I need some help! The aim was to detect the genotypes of various unknown samples with the H36D mutation using BCI I which abolishes the restriction site of a mutated individual. Our...

12 August 2015 6,279 2 View

Helicobacter pylori mutant knockouts with Kanamycin resistance growing on HBA + DENT + Kan plates but not appearing on gels or appearing as wild type?

Grown on 10% HBA + selective supplement dent DNA extraction (Qiagen) PCR: Wild Type + Mutant knockout samples: -8.5μl milli-q water -1μl forward primer corresponding to the gene that is knocked...

01 January 1970 6,950 4 View