Alexey Galashin

5 Questions 1 Answers 0 Followers

Questions related from Alexey Galashin

Why are brain slices kept at near-physiological temperatures immediately after cutting?

Many protocols for obtaining acute brain slices for electrophysiology involve cutting the brain in an ice-cold solution, then transferring the brain slices into a solution pre-warmed to...

31 May 2024 2,018 2 View

Can I carbogenate and freeze sucrose-based ACSF a day or even a few days before an experiment?

I'm studying hippocampal pyramidal and granule cells in coronal mouse brain slices using patch-clamp. I use mixed NaCl/sucrose ACSF for cutting (ice-cold) and recovery (30 min at 35-37 °C), 0.5...

31 May 2024 3,433 1 View

Does storing brain slices in sucrose-based ACSF vs. normal ACSF affect slice quality?

I've recently started to patch dentate gyrus granule cells in coronal mouse brain slices (300 μm). I'm using mixed NaCl/sucrose ACSF for cutting (ice-cold) and recovery (30 min at 35-37°C) and...

28 May 2024 3,652 1 View

How do I cut the mouse brain to obtain horizontal slices for patching hippocampal CA3 pyramidal cells?

I'd like to learn how to prepare horizontal mouse brain slices to patch CA3 pyramidal cells and, preferentially, CA1 pyramidal cells and DG granule cells. I've seen different protocols, and I'm...

20 November 2023 7,723 4 View

Why are CA3 pyramidal cells in my slices dead? Can their survival depend on the slicing plane?

I'm studying hippocampal pyramidal and granule cells in coronal mouse brain slices using patch-clamp. The mice are 2-3 months old. I've been able to record from CA1 pyramidal cells and DG granule...

20 November 2023 3,218 4 View