Alexandros S Frydas

2 Questions 4 Answers 0 Followers

Questions related from Alexandros S Frydas

Inconsistent results after plasmid re-purification from frozen stock?

Hello everyone, I am performing reporter gene assays on some uORF mutants vs my WT 5' UTR of my gene of interest. I am doing double transfection on HEK293T cells, using Nanoluc as a test reporter...

23 November 2020 2,833 5 View

What would be the most suitable blocking solution for streptavidin blotting?

Hello everyone! I am using BioID to detect some biotinylated proteins. I use streptavidin antibody, which is HRP conjugated. I have been experiencing background issues in general. I was blocking...

21 June 2017 9,678 4 View