Alexandria Nichols

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Questions related from Alexandria Nichols

If I am measuring the fluorescence of a substrate at 350nm ex but another compound in the reaction has an 287nm ex will that interfere?

I am running an enzyme assay that measures the fluorescence of a cleaved substrate at 360nm ex and 445em. I also have a compound in the reaction that can be fluorescent if excited at 287nm. Will...

09 October 2020 5,006 5 View

Any suggestions on how to achieve better yeast cell wall digestion using Zymolyase?

Our lab has been having issues digesting yeast cell walls with Zymolyase during cell fractionation. We use cultures with an OD of 0.6-0.7 and have increased the conc. of Zymolyase to 20mg/mL...

20 April 2018 1,710 3 View