Alexander Moysa

2 Questions 9 Answers 0 Followers

Questions related from Alexander Moysa

What is the optimal plasmid for protein expression?

I need that my protein after expression contains C-terminal His-tag and cleavage site before this His-tag (Tev protease / Thrombin ) without any addition signal sequenses and tags on the...

06 June 2016 1,175 4 View

How to get a monomeric form of RAGE protein?

Hi there, I try to get a membrane protein RAGE in monomeric/dimeric form. After overexpression e.coli and Ni-agarose purification I have a high order oligomer (~5*10^6 Da, light scattering...

02 February 2016 9,071 11 View