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Questions related from Alexander Malogolovkin
I wonder if it is possible to do a preliminary genetic characterization of phages using any universal primer set for following Sanger sequencing? We have several phages from Citrabacter, Protey,...
08 August 2017 8,708 2 View
We have got a recombinant protein which consist of target molecule and EGFP. However, the Mw of this fused protein is two times bigger than it was predicted. We are wondering if it could be a...
10 October 2015 7,375 12 View
We would run RT-qPCR to measure viral gene expression profile. In order to extract RNA from infected cell line Qiagen RNAeasy mini kit was used. However, big enough amount of DNA was still...
04 April 2015 5,911 15 View
Could anyone help me to define a range (in mg) of expressed protein in mammalian cell after transfection. We use a plasmid with CMV promoter and would like to know what is the values consider as...
10 October 2014 5,699 9 View
We have fused a protein of interest with EGFP into plasmid vector under CMV promoter control. In WB we have got two bands correspond to EGFP itself and fused recombinant protein according to Mw....
07 July 2014 435 9 View
We have an issue to amplify 3' end of dsDNA virus genome (ASFV). Basically, we use Long-Range PCR to amplify whole viral genome. Everything was fine except 3'end region. We still have around 6 kb...
04 April 2014 6,463 16 View
There is no doubt that widely-used Kaerber or Spearman or other methods based on virus back titration using 10- fold dilutions have shown acceptable results. Anyway, if we need to estimate virus...
07 July 2013 1,515 13 View
Currently working on glycoprotein structure (CD2v) in frame of prediction modeling. Given glycoprotein has a lot of glycosylation sites and our question is could the glycosylation affect Ab...
12 December 2012 7,595 2 View
