Alexander Soloniuk

2 Questions 1 Answers 0 Followers

Questions related from Alexander Soloniuk

Do RAW 264.7 cells express ASC, and thus NLRP3 Inflammasome activity?

I have been working with NLRP3 inflammasome signalling and have been using RAW 264.7 murine macrophage cells stimulated with LPS to do so. I have recently come across several sources asserting...

14 May 2019 7,234 2 View

I've been working with RAW 264.7 cells and am trying to activate the NLRP3 inflammasome. What are some methods that might achieve this reliably?

The results for activation I have been observing have been scattered and inconsistent. I am looking for reagents or a protocol to reliably induce NLRP3 inflammasome activation and show a clear...

06 March 2019 2,372 1 View