Alexander Moysa

2 Questions 7 Answers 0 Followers

Questions related from Alexander Moysa

What is the optimal plasmid for protein expression?

I need that my protein after expression contains C-terminal His-tag and cleavage site before this His-tag (Tev protease / Thrombin ) without any addition signal sequenses and tags on the...

25 June 2016 4,304 3 View

Does anyone have any experience with membrane protein reconstitution in nannodiscs?

My task is incorporation a RAGE protein into nanodiscs. I try to do it but results were not successful. After reconstitution nanodiscs I have a nice nanodiscs but it contains a small amount of...

15 March 2016 9,950 5 View