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We would run RT-qPCR to measure viral gene expression profile. In order to extract RNA from infected cell line Qiagen RNAeasy mini kit was used. However, big enough amount of DNA was still...
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We have fused a protein of interest with EGFP into plasmid vector under CMV promoter control. In WB we have got two bands correspond to EGFP itself and fused recombinant protein according to Mw....
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We have an issue to amplify 3' end of dsDNA virus genome (ASFV). Basically, we use Long-Range PCR to amplify whole viral genome. Everything was fine except 3'end region. We still have around 6 kb...
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