Alexander Malogolovkin

4 Questions 20 Answers 0 Followers

Questions related from Alexander Malogolovkin

How can I get rid off DNA for following with RT-qPCR?

We would run RT-qPCR to measure viral gene expression profile. In order to extract RNA from infected cell line Qiagen RNAeasy mini kit was used. However, big enough amount of DNA was still...

28 April 2015 8,797 14 View

Can EGFP fused proteins be expressed separately?

We have fused a protein of interest with EGFP into plasmid vector under CMV promoter control. In WB we have got two bands correspond to EGFP itself and fused recombinant protein according to Mw....

28 July 2014 4,573 8 View

Does anyone have an experience in 3' end viral genome amplification?

We have an issue to amplify 3' end of dsDNA virus genome (ASFV). Basically, we use Long-Range PCR to amplify whole viral genome. Everything was fine except 3'end region. We still have around 6 kb...

12 April 2014 8,807 15 View

Does anybody know how to predict antibody binding sites on protein molecules?

Currently working on glycoprotein structure (CD2v) in frame of prediction modeling. Given glycoprotein has a lot of glycosylation sites and our question is could the glycosylation affect Ab...

25 December 2012 4,549 2 View