Alexander Howard

3 Questions 4 Answers 0 Followers

Questions related from Alexander Howard

Why must an antibody be at a conc. of 1-10 mg/ml for reduction/conjugation reactions?

I am attempting to conjugate an antibody to a maleimide-drug conjugate. All the TCEP reduction protocols I find specify a protein concentration of 1-10 mg/ml in some sort of buffer (sodium borate,...

09 March 2018 5,014 1 View

Which substrate of cdk1 can be used to selectively measure its activity in vitro

I am looking to test various cdk inhibitors in cell lines and would like to be able to measure relative effects by following levels of phosphorylated target proteins. For example, the...

17 May 2017 6,550 3 View

Could somebody please help me to identify a self-immolative linker to connect a hydroxyl group to an amine? (many thanks in advance)

The purpose is to conjugate a drug via its hydroxyl group to an antibody. Following linker cleavage, it is important for the linker to self-immolate in order to restore the drug to its original...

08 February 2017 2,116 4 View