Alexander Howard

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Questions related from Alexander Howard

Why must an antibody be at a conc. of 1-10 mg/ml for reduction/conjugation reactions?

I am attempting to conjugate an antibody to a maleimide-drug conjugate. All the TCEP reduction protocols I find specify a protein concentration of 1-10 mg/ml in some sort of buffer (sodium borate,...

09 March 2018 4,968 1 View

Could somebody please help me to identify a self-immolative linker to connect a hydroxyl group to an amine? (many thanks in advance)

The purpose is to conjugate a drug via its hydroxyl group to an antibody. Following linker cleavage, it is important for the linker to self-immolate in order to restore the drug to its original...

08 February 2017 2,081 4 View