Using an expression vector, what explanation(s) could there be for an expressed protein to be 10kDa heavier than predicted? estimated from cDNA seq).

Hi, we sub cloned a DNA sequence into our vector of choice. The sequence subcloned was predicted to give a protein molecular weight of 36 kda. When western blot was performed of transduced cells (...

02 February 2012 6,049 10 View