Alex Jureka

5 Questions 3 Answers 0 Followers

Questions related from Alex Jureka

DE3 Bacterial cell lysis conditions in Branson SFX150 Sonicator?

Hi All, We are interested in using the Branson SFX150 sonicator we have in our lab to lyse DE3 E.coli in order to purify a couple of proteins. I have worked with larger sonicators in the past to...

05 May 2019 7,002 2 View

Will heat treating cell culture supernatants effect their performance in downstream applications such as ELISAs?

Hi everyone, I am currently trying to work out inactivation procedures for influenza virus. I am interested in using heat treatment for cell culture supernatants, but I'm concerned that heating a...

06 June 2018 4,138 3 View

Program for aligning protein sequences to reference sequence?

Hi everyone, I am currently trying to align several thousand (5-10) protein sequences to a related protein sequence (reference) to determine the percent identity of the large subset of sequences...

08 August 2014 6,319 11 View

How sensitive is FITC to heat and pH changes for the purposes of fluorescence anisotropy?

I have recently tried using fluorescence anisotropy to measure FITC-tagged shRNA binding to an RNA binding protein. My questions are: 1.) Will running an annealing program with high temperatures...

06 June 2014 6,346 6 View

Is it possible to do intra-cellular staining for only proteins in the cytoplasm (no permeabilization of the nuclear membrane) for flow cytometry?

I am currently interested in doing some flow cytometry to accompany confocal protein co-localization studies in order to get a more quantitative measure of the co-localization. The problem is, we...

05 May 2014 6,354 4 View