I have done a digest that should only linearize my plasmid, but I am getting 2 bands & I do not think it is supercoiled plasmid, any ideas?

I have done a digest with EcoR1that should only linearize my plasmid as it has a single cut site, but I am getting two bands & I do not think it is supercoiled plasmid. The top band is running...

13 December 2017 4,652 3 View