Alex Belardo

3 Questions 2 Answers 0 Followers

Questions related from Alex Belardo

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

29 January 2021 1,163 2 View

How can I fix issues with high salt concentration after gel purification?

I am using the 30-uL QIA gel purification kit, and each time I test the product on a nanodrop after, the 260/230 is in the 0.01-0.30 range. Subsequent reactions, like ligations have issues with...

23 September 2020 3,040 4 View

If I need to adjust settings for imaging+analysis, what is the "best" step to do so, from an experimental design standpoint?

I'm working on getting replicates of an ICC protocol and imaging+counting cells for analysis. I've noticed that, between replicates, there are differences in fluorescence, likely resulting from...

01 January 1970 9,430 4 View