Alessio Mallozzi

3 Questions 3 Answers 0 Followers

Questions related from Alessio Mallozzi

How do I design a construct to stably integrate in a cell line with two indipendent genes?

Hello everybody, I'm writing to ask a question concerning the stable integration of multiple gene into a cell line. In particular, I'm planning to exploit transposon based systems such as...

06 December 2022 4,054 4 View

Different AcrII4A protein efficiency after cloning? Can the RNA stability or the WPRE element make the difference?

Hello everybody, I would need help because I'm struggling a bit after cloning an Anti-CRISPR protein, AcrII4A. This protein is supposed to bind to the Cas9 protein and to inhibit it and I'm using...

27 August 2021 336 0 View

Genetically encoding 4 different fluorescent proteins?

Hello, I am planning an experiment and I would need to encode 4 different fluorescent proteins in mammalian cells, that should be seen and distinguished with a fluorescence microscope in a live...

31 January 2020 6,332 3 View