Alessio Mallozzi

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Questions related from Alessio Mallozzi

How do I design a construct to stably integrate in a cell line with two indipendent genes?

Hello everybody, I'm writing to ask a question concerning the stable integration of multiple gene into a cell line. In particular, I'm planning to exploit transposon based systems such as...

06 December 2022 4,050 4 View

Different AcrII4A protein efficiency after cloning? Can the RNA stability or the WPRE element make the difference?

Hello everybody, I would need help because I'm struggling a bit after cloning an Anti-CRISPR protein, AcrII4A. This protein is supposed to bind to the Cas9 protein and to inhibit it and I'm using...

27 August 2021 292 0 View