Alessandro Castorina

3 Questions 13 Answers 0 Followers

Questions related from Alessandro Castorina

Can the protein lysis buffer significantly alter ELISA measurements? What is the reagent responsible for such an effect?

We have performed IHC studies on gastric tissue to identify ghrelin expression before and after treatment and results were clear. Treatment increased immunoreactivity. To assess whether production...

21 September 2014 6,204 6 View

Electrophoretic mobility shift assay (EMSA) used to measure DNA binding activity requires radioactivity. Is there a non-radioactive approach?

Unfortunately, in our labs we are neither trained nor equipped for the radioactivity-based approach.

28 November 2013 578 8 View

How do you perform in vivo zymography? There are no detailed protocols online.

I would like to perform qualitative/quantitative analyses on hippocampal brain slices or in brain protein extracts to evaluate protease activity. I have read something about in vivo zymography,...

17 October 2013 479 15 View