Alessandra Gastaldello

5 Questions 7 Answers 0 Followers

Questions related from Alessandra Gastaldello

Which apoptosis genes to use for RT-qPCR.?

I would like to investigate the expression of some genes express in apoptotic cells by RT-qPCR in order to compare the results obtained by flow-cytometry (Annexin V). I didn't find in literature...

04 April 2014 4,763 10 View

Does someone have a good protocol for immunofluorescence of cell in suspension?

I have to do, for the first time, immunofluorescence on cells in suspension and just I don't know how to proceed.

01 January 2014 431 4 View

Can anyone help with protein extraction from rat testis cells?

Which is the best method to extract protein from cells in culture to do western blot analysis? In particular I am working with cells from rat testes and they are very hard to homogenize in...

11 November 2013 982 5 View

Best blocking buffer in immunofluorescence - can anyone help?

I work with rat testes primary culture and I am trying to characterize them by IF. I am using antibodies against GATA4, FSHreceptor, SyCP3, DDX4, HSD-3 beta, etc... but I am not able to have a...

10 October 2013 2,270 18 View

Concentration of DNase I for testis dissociation?

I have to dissociate rat (7days old) testis for obtaining Sertoli cells. I have the protocol and I have to use a solution of Collagenase IV and DNase I (M0303S BioLabs) in DMEM. My problem is that...

06 June 2013 3,435 5 View