Aleksej Drino

4 Questions 2 Answers 0 Followers

Questions related from Aleksej Drino

How to concentrate Ion Exchange fractions for Mass Spectrometry?

Hi, I am performing a purification of an complex of unknown constitution by following the enzyme activity, and one of the final steps is performing an Ion Exchange Chromatography. Now I want to...

06 June 2018 2,778 4 View

How to get rid of glycogen from RNA pellet?

I am performing some chromatography with RNA I precipitate using Ambion glycoblue. However, I see that the precipitant is hampering the column run by sequestering and changing the viscosity of the...

11 November 2017 8,904 0 View

Help with tRNA reverse transcription ?

I am trying to reverse transcribe tRNAs from primers annealing completely in the 3' regions of the tRNA. However, for some of them I can detect the cDNA, and for some of them I can not. I guess...

04 April 2017 8,216 4 View

How can I add a single stranded overhang on the 5' end of my PCR product?

03 March 2013 4,916 5 View