Alejandro Piña Iturbe

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Questions related from Alejandro Piña Iturbe

Why my transformed BL21(DE3) stops growing at OD600=0.55-0.6?

Hi everyone! Previously, I transformed a BL21(DE3) strain with a pET-15b vector carrying my gene of interest. The strain grew and I could express my protein. Now I need to do that again, but...

04 April 2019 2,942 5 View

Can I be sure that, after P22 transduction, the DNA region containing the resistance gene was the only one transferred?

Hello everyone! I've constructed a mutant strain of Salmonella using the lambda Red system (Datsenko & Wanner, 2000). After obtaining the resistant colonies and confirming insertion of the...

08 August 2017 5,853 0 View