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Hi there, I´ve tried to purify a GST-tagged protein using IPTG and GST-Sepharose beads. Although I have used several bacterial volumes (50.100.200 ml), differents IPTG concentrations (0.5 and 0.2...
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I am pursuiting to study the role of a miRNA in the expresion of a gene using luciferse assay. However, I´ve never transfected miRNAs before. I´ve read in other prior questions that PEI also works...
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