I'm doing a whole mount ISH for zebrafish embryos, and 2/5dpf are overstained (heads are very black/dark purple). Can bleaching overcome this?

I accidentally used the same staining times for 2, 3 and 5 dpfs. 3dpfs look good after bleaching with H2O2. 2 and 5 dpfs (especially 2 dpfs) have a very black head, and even after 6 hours in...

20 August 2016 721 1 View