Alejandro Armas

3 Questions 2 Answers 0 Followers

Questions related from Alejandro Armas

Why doesn't my cloning work when I use cloning primers? How many bp must be flanking the restriction sites in a cloning primer?

I want to clone a gene fragment of around 480 bp in the p-RSET-A vector. To do this, I designed two cloning primers that contain the Bamh I and Hind III restriction sites. The primers worked...

28 February 2024 6,711 3 View

Why do my ct drop when more sample preparation time has elapsed in RT-PCR?

We are testing a new reverse transcriptase in our lab for RT-qPCR and have noticed that within 15 minute time lapses the cts have started to increase for the low cts samples and the high ct's...

17 February 2023 9,924 1 View

I need to isolate pET28a+ plasmid from E. coli Rosseta, the issue is that this strain contains pRARE plasmid too. How should I proceed?

I have tried to separate the plasmids by extracting the DNA from agarose gel but I have only managed to make pRARE functional. I have been able to transform E. coli with pRARE that I obtained from...

12 December 2022 7,429 3 View