What is the best protocol to culture primary mouse lacrimal gland epithelial cells?

I'm culturing primary mouse lacrimal gland epithelial cells in DMEM-F12 supplemented with 10% FBS, 0,4 ug/ml hydrocortisone, 0.1 nm CT, sodium bicarbonate, adenina powder, tripsin, transferin,...

05 May 2017 8,703 2 View