Alden Richter

2 Questions 3 Answers 0 Followers

Questions related from Alden Richter

Protocol for transient transfection and expression of rAB?

Hi All, Does anyone have a good protocol for transient transfection of mammalian cells using separate plasmids for heavy and light chain of a recombinant antibody? What range of ratios should I...

01 October 2019 1,140 1 View

Troubleshooting rAb expression?

Hi All, I'm trying to express a rAb in CHO or HEK293 cells and I am having an issue getting functional antibody. WB shows bands for HC and LC under reducing conditions and non-reduced shows a...

11 July 2019 8,749 4 View