My protein is not getting expressed in E.coli. How can I overcome this?

Hi! I'm trying to express a human protein (GST-NTCP) in E.coli (BL21 cells). The protein is cloned in pGEX6P-1 vector and is 64KDa in size. After DNA cloning, the plasmid's sequence was correct so...

14 July 2016 5,431 3 View