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Questions related from Alan Moraes
I want to know how effective is the enzymatic lysis for recombinant protein extraction from E. coli. I saw in Sambrook and Russell their protocol. However, here at my departament we dont use it...
10 October 2015 9,980 3 View
Hi There, I'm working with a T.cruzi mitochondrion protein that has a 20 residues hydrophobic region. I'm using a CodonPlus strain and expressing it into a pET28a vector in LB media, 20ºC, 0.2 uM...
07 July 2015 8,817 3 View