Alan Harrison

3 Questions 11 Answers 0 Followers

Questions related from Alan Harrison

Could someone help me with interpreting Sybr Green I curves?

We recently ran an analysis of some samples using Sybr Green PCR. We have obtained some strange results for some of the samples. Two have a double peak in the melting curve, and low levels of...

09 September 2013 4,840 11 View

Any ideas what can cause inconsistency between the results of genus specific and species specific qPCR results?

I am using a genus specific qPCR for bacterial diagnosis and following up with a species specific qPCR to identify species within the genus. However, I am getting some samples that are negative...

05 May 2013 7,101 3 View

Anyone familiar with software or R code that would be suitable for power analysis of generalised linear mixed models?

Trying to set up some power analysis for infection status with nested factors.

03 March 2013 4,110 8 View