Alan Chan

4 Questions 10 Answers 0 Followers

Questions related from Alan Chan

Species identification in clinical sample?

I am doing some bacteria ID work in biological specimens which may contain different bacteria. Most of the time I cannot get pure culture and I just amplify DNA using genus/species specific...

04 April 2015 6,359 7 View

Can you do the qPCR presence/absence experiment using SYBR green?

I just found that the user guides of Stepone machines do not recommend the use of SYBR green for the qPCR absence/presence experiment, but I think that my melt curve analysis is good enough to...

11 November 2014 2,550 10 View

Why am I getting an abnormal qPCR amplification plot?

Hello everyone! I am using AB Stepone system with Qiagen QuantiNova PCR kit. After doing my first trial, my qPCR results were very strange, the plot are "hook"-shaped (attachment). Anyone know...

10 October 2014 7,248 7 View

How can I extract DNA of Gram positive bacteria from animal tissue?

Anyone can suggest kits for extracting bacterial DNA from animal tissue? I plan to extract bacterial DNA from swab using the following protocol: 1. Suspend swab in PBS 2. Centrifuge and obtain the...

10 October 2014 9,113 10 View