Alan Moraes

2 Questions 2 Answers 0 Followers

Questions related from Alan Moraes

Is there any protocol for defined media for macrophage cultures?

I want to perform an infectivity assay to test a metal compound and I need to guarantee that the medium hasn't it in a free form (apart from proteins). There is any protocol using a defined media...

20 January 2017 3,064 3 View

Has someone used enzymatic lysis for protein extraction as the sole lysis protocol?

I want to know how effective is the enzymatic lysis for recombinant protein extraction from E. coli. I saw in Sambrook and Russell their protocol. However, here at my departament we dont use it...

14 October 2015 707 3 View