I performe conventional PCR target 16srRNA gene of H.pylori , their is nonspesific band & fade target band 502 DNA 7mi anneling 63C exten72 C 2min ?

Intial denaturation 94C for7 min,denaturtion 94C 1min anneling63C for 1 min extention 72 for 2min DNAfrom gastric biopsy

02 February 2018 1,513 4 View