Al-Hassan M. Mustafa

3 Questions 4 Answers 0 Followers

Questions related from Al-Hassan M. Mustafa

Is it logic to get DNA double strand breaks but no single strand breaks?

I have started with COMET assay (Neutral and alkaline) and I got only results with neutral one but not the alkaline one. This has been done twice. Moreover, I have done also immunofluorescence...

28 August 2017 6,467 3 View

What could be the case, rescued cell death but still reduced protein levels?

Cell death was rescued by 50 μM Z-VAD but I still have destabilization of the phosphorylated form of the oncoprotein (simply as clear as treated band) in Western blot. I have also tried with...

17 July 2017 9,634 5 View

Is the 100 uM Z-VAD-FMK is acceptable to be used for the leukemic cells as a pan-caspase inhibitor?

I have used 50 uM to block capases. This concentration was successful to block apoptosis in the sub G1 analysis when it was oretreated one hour before my drug, however, I have detected also a cl....

21 October 2016 5,927 0 View