Akshay Tharali

3 Questions 1 Answers 0 Followers

Questions related from Akshay Tharali

How to increase RNA yield and purity from PBMCs stored in RNA later at -80 C?

I have been using miRNA Isolation mini kit (qiagen 217004) for isolating RNA from PBMCs stored in RNA later at -80 C. The cell no. was 2 million cells. The RNA yields obtained are around 30-40...

16 October 2019 4,881 1 View

How do I convert absorbance readings for amylase activity assay into units/ml?

I already have plotted a maltose standard curve but the readings for absorbance of amylase activity assay are beyond the range of the maltose standard curve. The abosrbance reading for 1.8mg of...

16 May 2016 780 6 View

Concentration of methylene blue in Microbial fuel cell?

What should be the concentration of mmethylene blue that should be added as an electron mediator in a MFC of volume 700ml? Also how to dissolve it as it settles down after some time?

28 April 2016 8,466 1 View