Akshay Pareek

9 Questions 7 Answers 0 Followers

Questions related from Akshay Pareek

Low ipTM score in alphafold models. How to improve?

Hello everyone, I am trying to do protein-protein modelling using alphafold. So I am getting very loe ipTM score (0.2). So I want to know that how to improve these models and how to confirm the...

26 November 2024 5,271 0 View

Any expereince about gluteraldehyde crosslinking?

I am trying to crosslink my protein by gluteraldehyde but after crosslinking multimeric form of protein is not able to run on SDS PAGE even after 6 hrs long run at 90V. Please share your experience.

21 March 2018 769 3 View

RE digestion of plasmid

I am trying to digest a plasmid having 1.5 kb insert with NdeI and XhoI but I see only a single band at large size. Plasmid was expected to having these restriction sites. So what can be the...

18 July 2017 4,634 3 View

Size of plasmid?

I have transformed cloned plasmid in DH5a cells and isolated plasmid from it. when I run gel, expected band should be on 5 kb but it is around 10-11 kb. I cant sort out the reason for this. Is it...

07 March 2017 6,140 2 View

Problem with PCR amplification ?

I am trying to amplify a gene in same conditions (i.e. melting temp, annealing temp and extension temp and time for all steps) on which I have previously amplified this gene. But this time I am...

22 January 2017 7,565 3 View

Rosetta cells in autoinduction media?

Hello I have cultured rosetta cells in ZYP5052 autoinduction media. It is showing very slow growth. Does anyone have some experience with this media? please share .

14 December 2016 9,656 3 View

Does type III restriction enzyme also require few nucleotides ahead of primer sequence ?

I am designing a primer for a gene having BsaI restriction site. Do I need to include 3-4 base ahead of recognition site for enzyme to bind.

11 December 2016 2,817 3 View

Restriction sites known but getting unknown gel band. How?

I digested my circular plasmid having 1 RE site for BamH1 and 1 for Bsa1. So I supposed to get 2 bands but getting one more low molecular weight band. Please explain any reason.

07 December 2016 1,861 14 View

Solubility of Protein on lower temperature?

I have read in experimental procedure that lowering the temperature will translate more soluble protein. What is the reason behind it ?

18 August 2016 3,311 8 View