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How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.
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We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.
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I have used collagenase type I, followed by trypsin to isolate Wharton's jelly derived MSCs, but we still couldn't see any cells. Do you have any suggestion to optimize this experiment?
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