Can anybody tell me if ortho-phosphoric acid present in destaining solution interferes with the trypsin digestion step in MALDI due to its low pH?

I have destained my 2D gel with a solution containing 10% ortho-phosphoric acid and10% methanol. However I cannot see any peptide in the MALDI spectrum. Can it be possible that the low pH of...

06 December 2016 6,748 3 View